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It finally subsided once I broke out in a sweat and apparently my fever was gone and so was the arm pain

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Select Flavor ::Caribbean Breeze

The cells were again washed thrice with PBS and incubated with the appropriate combination of secondary antibodies (1: 400, Alexa Fluor 488-conjugated goat anti-mouse or anti-rabbit and Alexa Fluor 549-conjugated goat anti-mouse or anti-rabbit secondary antibodies