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For instance, peptides containing methionine, cysteine, or tryptophan residues are more prone to oxidation
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Allow to separate, draw off the lower layer into a second small separator, add 5 mL of 5 N sulfuric acid, shake well, and allow to separate completely (the complete separation of the layer may be facilitated by centrifuging): the separated upper layer is colorless or has no more color than a mixture of 0.15 mL of 0.10 N potassium permanganate and 250 mL of water
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