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Lyophilised Dihexa stored at −20°C maintains structural integrity for 12–24 months, but once reconstituted with bacteriostatic water, the solution must be refrigerated at 2–8°C and used within 28 days

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(2) molecular dynamics simulated annealing restraining FOXO4 FH (without flexible regions) harmonically to its template structure (using a non-crystallographic force constant of 10,000 kcal mol −1 Å −2 ), with additional dihedral angle restraints from 1 H, 15 N, and 13 C chemical shifts using TALOS-N 56 , hydrogen bond restraints (region p53 residues 48-55