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Chemical method usually redundantly biotinylates a protein (left), whereas the enzymatic method specifically biotinylates a protein on its Avi-tag (right So, in cases where there is more than one cysteine present in a protein, researchers will often mutate out cysteine residues until there is only one left
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Future studies should focus on individualized supplementation based on PCOS phenotype, genetic background and nutrient-nutrient interactions ( Table 1 )
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